ValidPrime Validprime kit:ValidPrime® Mouse – SYBR assay – 250 rxns Designed for SYBR® Green-based qPCR

Designed for SYBR® Green-based qPCR and probe based qPCR

From the combined measurements of the ValidPrime® assay and the gene of interest (GOI) assays on all samples and on the gDNA control

There is no universal control gene

c endonuclease is active over a broad pH range and displays optimum activity at salt concentrations around 175 mM

study-specific housekeeping gene

ValidPrime Validprime kit:ValidPrime® Mouse – SYBR assay – 250 rxns Designed for SYBR® Green-based qPCRValidPrime replaces the need to perform no reverse transcriptase (RT( )) controls to test for the presence of genomic DNA (gDNA) for all samples in your real time quantitative PCR (qPCR) profiling. In an expression profiling experiment based on m samples and n assays, traditional set up requires m RT( ) reactions plus m x n qPCR controls. When using ValidPrime only m + n + 1 controls are needed. ValidPrime will minimize the amount of control reactions