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98 Agar 15 Final pH 7
HL5 supplemented with vitamins and micro-elements as present in FM and SIH Media
LoFlo vs VLoFlo: Triplicate biological repeats (cultures) Cell counts made to 48 h Note: by 26 h cells round and shrink* in both LoFlo and VLoFlo By 47 h cell rounding and shrinkage pronounced and assay stopped * Noted previously in Dicty community that LoFlo is not ideal for long-term culturing
Weight 244
Glucose 20 Potato extract 4 Suspend 24 gram powdered medium in 1 litre distilled water
SC Broth / 3.3% Succinate Size:250g 98 Agar 15 Final pHSuspend 41. 9 gram powdered medium in 1 litre distilled water Store dry at room temperature